Size Reduction Efficiency of Alpha-Mangostin Suspension Using High-Pressure Homogenization

assessment of the efficiency of s.p.g.c refineries using network dea

data envelopment analysis (dea) is a powerful tool for measuring relative efficiency of organizational units referred to as decision making units (dmus). in most cases dmus have network structures with internal linking activities. traditional dea models, however, consider dmus as black boxes with no regard to their linking activities and therefore do not provide decision makers with the reasons...

Preparing Kaempferol Nanosuspension (KNS) using High Pressure Homogenization (HPH) technique

Background During the last decades, researchers have provided a plethora of therapeutic uses of kaempferol, including anticancer, anti-inflammatory, neuroprotective, anti-oxidative and anti-oestrogenic activities, making kaempferol a valuable compound [1]. However, being a poor water-soluble compound, kaempferol often has insufficient solubility and bioavailability [2].To find a solution for th...

High-efficiency transfection of suspension cell lines.

Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg white coatings with commercially available transfection reagents leads to high t...

A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

BACKGROUND Cell disruption is a key unit operation to make valuable, intracellular target products accessible for further downstream unit operations. Independent of the applied cell disruption method, each cell disruption process must be evaluated with respect to disruption efficiency and potential product loss. Current state-of-the-art methods, like measuring the total amount of released prote...

Microbial Cell Disruption by High-Pressure Homogenization